The interaction between human beings and nature in early phase mainly happened between relative isolated species and natural environment and the formation and development of culture were mainly restricted and influenced by natural environment. However, with the rapid development of transportation, economy and technology,etc; it is universal for different cultures blending and penetrating each other. However Qinghai plateau forms unique cultural phenomenon for its relative isolated physical environment. In the interior of Qinghai plateau, the effects of prevention and separation of huge mountains make the spatial variation of cultural distribution distinctly and the blending and penetration of various cultures are not wide, so the modern culture in this area still remains the special features of the early culture. The basic distributive pattern of mountains in Qinghai plateau forms different mountain cultures:Kunlun Mountain culture formed in the lak e basin between Qilian Mountain and Kunlun Mountain with the crossing region between the two mountains as the core region of this culture; mountain cultural region, Located in the northwest of Qilian Mountain, is a multi-nationalities integrating area. Hehuang valley area, the southeast of Qilian Mountain, is one of the main cradles of Chinese cultures; The Tibet cultural area with unique characteristics formed between Kunlun Mountain and Tanggula Mountain is named Tanggula Mountain culture area,which is divided into two sub-culture regions (Kangba culture area and Anduo culture area) by the line of Bayankela Mountain. By making a preliminary research on mountains distributive pattern and spatial variation of culture in Qinghai,this paper aims at revealing Qinghai cultural system formed in the environment of multi-nationalities and provides necessary background information for the development of economy and the construction of culture of Qinghai.