The spatial structure of urban-rural cultures includes that the cultural elements own their relative location and relationship, their interaction, mutual adaptation, the degree of agglomeration, and relative equilibrium relation between urban and rural areas in space. First and foremost, following the generalization that cultural information usually flows from the higher-grade nodes to lower-grade ones and the basic theoretical orientation of modern cultural communication, we manage to construct the tuberous measure index system for urban-rural cultural nodes in Wuhan city circle according to the representation of cultural information flow. Finally the tuberosity of the cultural nodes based on the spatial buffer and spline curve is analyzed with ArcGIS. From the view of point, line and surface, the paper demonstrates the evolution of the spatial structure of urban-rural cultures in Wuhan city circle under the information background. Three characteristics and regular findings are as follows:(1) there is hierarchical system with four grades in the urban-rural cultural nodes, which represents that the first-grade and the second-grade cultural nodes generally distributes spatially like a cross-shaped pattern along the Beijing-Guangzhou Railway and Yangtze River Economic Zones, while the third-grade and the fourth-grade ones are located in the quadrants of the cross-shaped pattern; (2) the outline of the cultural corridors is increasingly apparent; (3) the spatial structure of urban-rural cultures presents the tendency of network, while the signs of cultural agglomeration start to appear.