With the development of rural tourism and homestays, increasing numbers of women are choosing to settle in rural areas to establish new careers and identities. Studying the identity construction of rural female homestay owners can enrich the theory of identity construction from a gender perspective. This research focuses on rural female homestay owners in Shennongjia and employs qualitative methods such as participant observation and in-depth interviews to construct a three-stage model of their identity construction process: identity reconstruction, identity balance, and identity recognition. The research finds that the identity crisis and identity needs arising from the villagers' and femal identities drive the expansion of the homestay owners' identity under the joint influence of identity opportunities. Through the integration of multiple roles as "mother and wife, " "villager, " and "business operator" within a multi-dimensional space, female homestay owners achieve identity balance. Self-identity and social-identity are important indicators for successfully constructing the identity of rural female homestay owners. The research provides an empirical framework for gender studies in rural tourism and has practical implications for optimizing policies for women's entrepreneurship, activating endogenous development in rural areas and promoting rural revitalization.