Grounded in Lefebvre's theory of spatial production and drawing on new materialism and assemblage theory, this study adopts a qualitative approach to examine the production of everyday labor space of traditional marine aquaculture fishermen on Guanglu Island, Changhai County, Dalian. Viewing space as the outcome of continuous interactions between human and nonhuman elements, the research reveals how natural forces, cultured organisms, labor tools, and temporary workers collectively construct and constantly reshape the fishermen's working space. Methodologically, this study combines field observation with semi-structured interviews. The findings indicate that fishermen's labor space manifests in three interrelated dimensions. First, the physical space exhibits dynamic assemblage characteristics. Fishermen's work extends across the sea-land boundary, where diverse elements—humans, tools, cultured species, and natural environments—interact to form flexible, adaptive, and constantly changing spatial configurations. Second, the relational space embodies multiple tensions. The rigid demands of the marine environment and cultured species impose structural constraints, while the intervention of market and technological forces further intensifies these pressures. Within this unequal framework, fishermen rely on limited agency to negotiate contradictions and maintain production. Third, the subjective space reflects both physical and psychological predicaments. Long-term exposure to harsh environments leads to chronic pain and physical exhaustion, while repetitive work and economic uncertainty give rise to emotional numbness and a sense of helplessness.